Pysam
원문: pysam
Genomic file toolkit. Read/write SAM/BAM/CRAM alignments, VCF/BCF variants, FASTA/FASTQ sequences, extract regions, calculate coverage, for NGS data processing pipelines.
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Pysam Overview Pysam is a Python module for reading, manipulating, and writing genomic datasets. Read/write SAM/BAM/CRAM alignment files, VCF/BCF variant files, and FASTA/FASTQ sequences with a Pythonic interface to htslib. Query tabix indexed files, perform pileup analysis for coverage, and execute samtools/bcftools commands. When to Use This Skill This skill should be used when: Working with sequencing alignment files (BAM/CRAM) Analyzing genetic variants (VCF/BCF) Extracting reference sequences or gene regions Processing raw sequencing data (FASTQ) Calculating coverage or read depth Implementing bioinformatics analysis pipelines Quality control of sequencing data Variant calling and annot…
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